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Established A Index Adapters Seek advice from Index 2 (i5) Orientation For more info on how to enter i5 bases on the sample sheet in ahead or reverse complement orientation.

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When employing LRM, i5 sequences need to be entered into these software package from the forward orientation in all situations and for all instrument kinds.

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Index 2: the i5 index column is while in the reverse complement orientation (tend to be the reverse enhance of the i5 indexes inside the sample sheet).

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When assessing index sets for shade equilibrium, the sequence observed through the instrument must be assessed, regardless of the orientation employed for the sample sheet. With the i7 index, usually asses shade equilibrium utilizing the "i7 bases for sample sheet" column orientation.

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